English

Pasteur staining nuclear staining process (attaching glass slides)

Created on 06.15
Pasteur staining nuclear staining process (attaching glass slides)
  1. The soaking time of hematoxylin solution is generally 3-5 minutes, but it is necessary to adjust it according to the temperature and dye conditions. In summer or when using hematoxylin dye that has been stored for a long time, the coloring time should be shortened; Winter or newly prepared hematoxylin dye solution and diluted hematoxylin dye solution that has been used for a long time are not easy to color, and the time should be extended. There are generally two methods for using hematoxylin staining:
  1. Overstaining method: Firstly, consciously perform deep staining, and then use hydrochloric acid acidification process to make the nuclear staining more suitable. This method can remove the remaining hematoxylin dye adhered to the cytoplasm during the acidification process, making the cytoplasm staining more vivid, clear, and commonly used for specimens with high mucus content.
  2. Light staining method: In the process of nuclear staining, the staining time should be strictly controlled to ensure appropriate nuclear staining without the need for hydrochloric acid acidification. However, a small amount of hematoxylin in the cytoplasm can affect the quality of EA staining. Mainly used for specimens with less mucus to avoid cell detachment in large patches during acidification and tap water flushing processes. When using hematoxylin staining, it is necessary to filter it daily, otherwise the crystallization of hematoxylin will affect the quality of staining. Generally, hematoxylin dye can be used for a long time, so adding a small amount of fresh dye daily is sufficient.
2. Alkalization, also known as the process of returning to blue, can be achieved by using lithium carbonate or 3% ammonia solution for alkalization, with the aim of early color development of hematoxylin, which takes about a few seconds. More importantly, it is washed away by flowing water, which can make the blue color appear more vibrant. Alkaline solutions also need to be thoroughly rinsed to avoid hindering the next step of cytoplasmic coloring and color preservation after specimen preparation. The differentiation and alkalization solutions should be replaced with new solutions at least daily.
Contact
Leave your information and we will contact you.

Tel：+86 18751337033        +86 13656277722

Add：No. 11 Hongyang Road, Sanhe Town IndustrialZone, Haimen City, Jiangsu

0fe9f4d1df156efe69faf6b9cb6e5017.jpg