7109 Paint Slices: How to Stain with Pasteur Staining Solution

7109 Paint Slices: How to Stain with Pasteur Staining Solution

Wash away.

3. Immerse the smear in 1% hydrochloric acid alcohol staining solution for several seconds, wash with water and soak for 2-5

Minute blueing (can also be quickly reversed by dilute lithium carbonate).

4. Immerse the smear in 50% ethanol for 30 seconds and 95% ethanol for 2 minutes.

5. Soak the smear in orange yellow staining solution for 1-3 minutes.

6. Soak the smear twice in 95% ethanol

7. Soak the smear in EA50 staining solution for 5 minutes.

8. Soak the smear in 95% ethanol 2-3 times, dehydrate with anhydrous ethanol, and add xylene

Tongming, sealed with gum.

9. Microscopic observation.