Allow the test device and specimens to equilibrate to room temperature (15-30℃ or 59-86℉) prior to testing.

1. Remove the test device from the sealed pouch and use it as soon as possible.

2. Place the test device on a clean and level surface.

3. For serum or plasma specimen: Hold the dropper vertically and transfer 3 drops of serum or plasma (approximately 100μL) to the specimen well (S) of the test device, then start the timer. See illustration below.

4. For whole blood specimens: Hold the dropper vertically and transfer 1 drop of whole blood (approximately 35μL) to the specimen well(S) of the test device, then add 2 drops of buffer (approximately 70μL) and start the timer. See illustration below.

5. Wait for the colored line(s) to appear. Read results at 10 minutes. Do not interpret the result after 20 minutes.