Before testing, allow the test, specimen and/or controls to reach room temperature 15-30℃.

1. Bring the pouch to room temperature before opening it. Remove the test cassette from the sealed pouch and use it within one hour.

2. Place the cassette on a clean and level surface.

For Serum, Plasma:

Hold the dropper vertically, transfer 3 drops of serum or plasma (approximately 75 μL) to the specimen well (S) of test cassette and then start the timer. See illustration below.

For Venipuncture Whole Blood:

Hold the dropper vertically and transfer 3 drops of venipuncture whole blood (approximately 75 μL) to the specimen well (S) of test cassette, then add 1 drop of buffer (approximately 40 μL) and start the timer. See illustration below.

For Fingerstick Whole Blood specimen:

To use a capillary tube: Fill the capillary tube and transfer approximately 75 μL of fingerstick whole blood specimen to the specimen well of test cassette, then add 1 drop of buffer (approximately 40 μL) and start the timer. See illustration below.

3. Wait for the colored line(s) to appear. The result should be read at 10 minutes. Do not interpret the result after 30 minutes.